The nanoMedical Diagnostics (NMD) technology overcomes inherent limitations of mass spectrometry (MS) by separating (“fractionating”) relatively large and abundant serum proteins and other matter that have little clinical significance from circulating peptides and protein fragments (possible “biomarkers”) that provide diagnostic potential. This innovative approach provides a relatively rapid, economical, and high throughput method to detect early, active, and latent forms of the disease. The NMD diagnostic technology relatively enriches the target biomarkers from less than a single drop of blood. Quickly and physically removing large molecules from the sample, allows the mass spectrometer to operate at its most sensitive settings.
NMD Procedure Summary:
Step 1: Serum is collected from a small blood sample.
Step 2: The serum is dropped into a well of a NMD chip – Figure (i) below.
Step 3: The large proteins and other matter, which have insignificant diagnostic value, are unable to enter the nanopores of the NMD Chip.
Step 4: Low molecular weight proteins and peptides which provide diagnostic potential, are captured within the pores of the NMD Chip – Figure (ii).
Step 5: [Optional] A digestive enzyme is added to break down the captured proteins further to enhance detection – Figure (iii).
Step 6: The protein fragments are eluted from the NMD Chip and analyzed by mass spectrometry (MS) – Figure (iv).